Day 36: Out of My Mind
I'm officially sick. I can't think straight.
I hate not being to get any PCR products. Not once, but twice. How can that be possible? Now, I'm sick in more ways than one. Sick of being unable to amplify DNA.
In spite of my delirium, I have to buck up and get some results from the PCR process. Being unsuccessful twice is too much. I have to get some amplicons out tomorrow. I still need to purify them, measure the concentration, and dilute the products so that each has the same concentration. This perpetual tiredness of mine is not helping.
To all who emailed me, thanks for your concern. I'm recovering well. Tired, but not dead. (Do you think a smiley face at this point will look weird? Yah, I think so too.)
On the bright side, Ben is here to squat with me for a while. Though he won't be around too much, I think. His main aim is still to travel as much as he can on his sabatical. And my secondary school and JC friends will be here for the weekend. Together. But only the weekend. Shucks. It's amazing how they'd been able to obtain leave for the same dates. Just amazing. And they are coming together. Shucks, shouldn't have made the introduction some time back. Otherwise, I could have two groups visiting on different days.
I hope I'll be well enough to go skating on Friday. Hopefully there will be good weather too.
To my quick recovery, and a great weekend!
posted by Reborn @ 10:29 pm
5 Comments:
You better take somemore medicine. If condition still doesn't improve, then seeing a doc will be a good choice.
As for PCR, u are doing the 16S amplification?
maybe you can run a gel elect. to check conc. of your DNA again. If band is too thick, reduce concentration during PCR. Based on past experience, if you are doing 16S amp,it is unlikely that you have to change any of the annealing or extension temperatures. Varying template concentration should do the trick.
Good Luck!
Meanwhile, Rest well and get well soon!
Take care.
Thanks for your concern. I'm feeling much better.
And there's nothing wrong with my PCR now. Problem solved.
And no. I'm not amplifying 16S. This is old news, and too slow for our goal of fast community fingerprinting.
Anyhow, I received tons (I'm exaggerating, of course) of email about my health and experiments.
I would just like to state for the record that things are fine now. Thank you, everyone, for your concern and assistance.
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